The molecular weights here are derived from the 1997 IUPAC standard atomic weights, assuming pH = 7.0. The isoelectric points are calculated using the algorithm from ExPASy's Compute pI/Mw program, which was kindly provided by Elisabeth Gasteiger (see also Bjellqvist, B., Hughes, G.J., Pasquali, Ch., Paquet, N., Ravier, F., Sanchez, J.-Ch., Frutiger, S. & Hochstrasser, D.F., The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences, Electrophoresis 1993, 14:1023-1031). We have added the option to include phosphorylations, using pKa = 2.12 for the first ionization and pKa = 7.21 for the second.
| # Phosphates | Molecular Weight | Isoelectric Point |
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